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Table 4 Cutoff values for Ion Torrent PGM sequencing and TVC parameters

From: Quality control material for the detection of somatic mutations in fixed clinical specimens by next-generation sequencing

  Mean S.D. Confidence Level z Cutoff (at z-score)
Coverage 1231 526 95 % 199 200
Number of ≥ Q20 bases 2.5E + 07 2.4E + 06 95 % 2.0E + 07 2.0E + 07
Quality (log10) 3.65 0.45 99 % 2.48 300a
Strand Bias 0.7 0.04 99 % 0.79 0.79
  1. S.D., standard deviation; a, anti-logarithm of the found z value