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Table 4 Cutoff values for Ion Torrent PGM sequencing and TVC parameters

From: Quality control material for the detection of somatic mutations in fixed clinical specimens by next-generation sequencing

 

Mean

S.D.

Confidence Level

z

Cutoff (at z-score)

Coverage

1231

526

95 %

199

200

Number of ≥ Q20 bases

2.5E + 07

2.4E + 06

95 %

2.0E + 07

2.0E + 07

Quality (log10)

3.65

0.45

99 %

2.48

300a

Strand Bias

0.7

0.04

99 %

0.79

0.79

  1. S.D., standard deviation; a, anti-logarithm of the found z value