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Table 5 Clinical specimen results for known KRAS, EGFR and BRAF mutations

From: Quality control material for the detection of somatic mutations in fixed clinical specimens by next-generation sequencing

  KRAS or EGFR or BRAF Mutation Status
Sample_ID Single-Gene Assay CHP2 Assay
DNA_01 EGFR c.2369C > T (p.Thr790Met) EGFR c.2369C > T (p.Thr790Met)
DNA_02 EGFR c.2156G > C p.Gly719Ala EGFR c.2156G > C p.Gly719Ala
DNA_03 Negative Negative
DNA_04 Negative Negative
DNA_05 EGFR Exon 20 INS EGFR c.2307_2308ins9 (p.V769_D770insASV)
DNA_06 Negative Negative
DNA_07 Negative Negative
DNA_08 EGFR c.2369C > T (p.Thr790Met) EGFR c.2369C > T (p.Thr790Met)
DNA_09 EGFR c.2369C > T (p.Thr790Met) EGFR c.2369C > T (p.Thr790Met)
DNA_10 EGFR Exon 19 DEL EGFR c.2236_2250del15 (p.E746_A750delELREA)
DNA_11 Negative Negative
DNA_12 Negative Negative
DNA_13 Negative Negative
DNA_14 Negative Negative
DNA_15 Negative Negative
DNA_16 Negative Negative
DNA_17 Negative Negative
DNA_18 Negative Negative
DNA_19 Negative Negative
DNA_20 Negative Negative
DNA_21 KRAS c.35G > T (p.Gly12Val) KRAS c.35G > T (p.Gly12Val)
DNA_22 Negative Negative
DNA_23 EGFR c.2582 T > A (p.Leu861Gln) EGFR c.2582 T > A (p.Leu861Gln)
DNA_24 EGFR c.2582 T > A (p.Leu861Gln) EGFR c.2582 T > A (p.Leu861Gln)
DNA_25 Negative Negative
DNA_26 BRAF c.1799 T > A (p.Val600Glu) BRAF c.1799 T > A (p.Val600Glu)
DNA_27 KRAS c.34G > A (p.Gly12Ser) c.34G > A (p.Gly12Ser)
DNA_28 Negative Negative
DNA_29 EGFR c.2155G > A (p.Gly719Ser) EGFR c.2155G > A (p.Gly719Ser)
DNA_30 Negative Negative
DNA_31 Negative Negative
DNA_32 KRAS c.34G > T (p.Gly12Cys) c.34G > T (p.Gly12Cys)
DNA_33 BRAF c.1798_1799delGTinsAA (p.Val600Lys) BRAF c.1798_1799delGTinsAA (p.Val600Lys)
DNA_34 BRAF c.1798_1799delGTinsAA (p.Val600Lys) BRAF c.1798_1799delGTinsAA (p.Val600Lys)
DNA_35 EGFR Exon 19 DEL EGFR c.2235_2246del12 (p.Glu746_Glu749del)
DNA_36 Negative Negative
DNA_37 EGFR c.2573 T > G (p.Leu858Arg) EGFR c.2573 T > G (p.Leu858Arg)
DNA_38 EGFR c.2573 T > G (p.Leu858Arg) EGFR c.2573 T > G (p.Leu858Arg)
DNA_39 KRAS c.34G > T (p.Gly12Cys) c.34G > T (p.Gly12Cys)
DNA_40 Negative Negative
DNA_41 KRAS c.38G > A (p.Gly13Asp) c.38G > A (p.Gly13Asp)
DNA_42 KRAS c.38G > A (p.Gly13Asp) c.38G > A (p.Gly13Asp)
DNA_43 EGFR c.2573 T > G (p.Leu858Arg) Negative
DNA_44 EGFR Exon 20 INS c.2312_2314dupACC (p.Pro772delinsHisPro)
DNA_45 Negative Negative
DNA_46 Negative Negative
DNA_47 KRAS c.35G > T (p.Gly12Val) c.35G > T (p.Gly12Val)
DNA_48 Negative Negative
DNA_49 Negative Negative
DNA_50 BRAF c.1799 T > A (p.V600Glu) c.1799 T > A (p.V600Glu)
DNA_51 KRAS c.35G > A (p.Gly12Asp) c.35G > A (p.Gly12Asp)
DNA_52 BRAF c.1799 T > A (p.Val600Glu) BRAF c.1799 T > A (p.Val600Glu)
DNA_53 KRAS c.35G > T (p.Gly12Val) KRAS c.35G > T (p.Gly12Val)
DNA_54 EGFR c.2573 T > G (p.Leu858Arg) EGFR c.2573 T > G (p.Leu858Arg)
DNA_55 BRAF c.1799 T > A (p.Val600Glu) BRAF c.1799 T > A (p.Val600Glu)