Table 1 Features of the antibodies used for the immunohistochemical stains
Antigen | Clone, dilution, source |
|---|---|
Pan-Cytokeratin | AE1/AE3, 1:200, Leica, Newcastle/UK |
Epithelial Membrane Antigen | E29, 1:300, Biocare, Concord/CA |
PAX8 | PAX8, 1:100, Biocare, Concord/CA |
WT1 | 6 F-H2, 1:40, DBS, Pleasenton/CA |
ER | EP1, 1:100, Genemed, San Francisco/CA |
PR | SP2, 1:1000, Thermo, Fremont/CA |
CD34 | QBEND/10, 1:100, DBS, Pleasenton/CA |
SMA | 1A4, 1:1000, NeoMarkers, Fremont/CA |
Desmin | D33, 1:50, Biocare, Concord/CA |
S100 protein | Z0311, 1:6000, DAKO, Glostrup/Denmark |
GFAP | GA-5, 1:50, Thermo, Fremont/CA |
Bcl2 | Bcl-2-100, 1:80, Invitrogen, Paisley/UK |
CD99 | HO36-1.1, 1:100, Thermo, Fremont/CA |
MUC4 | 1G8, 1:50, Invitrogen, Paisley/UK |
HMB45 | HMB45, 1:25, DBS, Pleasenton/CA |
Melan-A | A103, 1:100, Thermo, Fremont/CA |
INI1 | 25, 1:50, Zeta, Arcadia/CA |