Table 1 Antibodies used and technique details
From: Four cases of cell cannibalism in highly malignant feline and canine tumors
Antigen | Clone/Source | Retrieval | Dilution, incubation time | Detection system/Source |
|---|---|---|---|---|
Pancytokeratin | Clone AE1/AE3 (DAKO) | Microwave 900W 5 min + 600W 15 min (Tris EDTA pH 9.0, Novocastra™ Epitope Retrieval Solution, Leica Biosystems, Wetzlar, Germany | 1:100 60min RT | Novolink (Leica Biosystems) |
Vimentin | Clone V9 (DAKO) | Microwave 900W 15 min (Tris EDTA pH 9.0, Novocastra™ Epitope Retrieval Solution, Leica Biosystems, Wetzlar, Germany) | 1:50 60 min RT | Novolink (Leica Biosystems) |
E-cadherin | Clone 36 (Ventana, Tucson, USA) | Microwave 600W 15 min (citrate buffer pH 6.0) | Ready to Use 60 min RT | Novolink (Leica Biosystems) |