Fig. 2
From: miR-449a targets Flot2 and inhibits gastric cancer invasion by inhibiting TGF-β-mediated EMT
miR-449a directly targeted Flot2. a Representative diagram of the predicted wild-type (WT) or mutant (Mut) binding site of miR-449a in the 3'- untranslated region (UTR) of Flot2 mRNA. The luciferase reporter plasmid containing the WT or Mut Flot2 3′-UTR was cotransfected into HEK293T cells with miR-449a mimics. Luciferase activity of the cells was assayed at 48 h after transfection, and the values were normalized to the normal control values. *P <0.01 (compared with the control). b qRT-PCR analysis revealed the effects of Flot2 siRNA and miR-449a mimics on the expression level of miR-449a. c Western blot analysis revealed the effects of Flot2 siRNA-810 and miR-449a mimics on the expression level of Flot2. Error bars represent ± S.E. and *, p < 0.01 versus control and NC