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Fig. 2 | Diagnostic Pathology

Fig. 2

From: RETRACTED ARTICLE: Protective role of silymarin in a mouse model of renal Ischemia–Reperfusion injury

Fig. 2

Effect of silymarin on renal tissue damage and cellular architecture after renal I/R injury. Renal histologic changes in the outer medulla from mice of sham, silymarin, I/R and silymarin + I/R groups were assessed using periodic acid–Schiff (PAS) staining. Representative slides from each group (original magnification ×400) are shown. a Histologic damage in the outer medulla was scored by counting the percentage of tubules that displayed tubular necrosis, cast formation and tubular dilation as follows: 0, normal; 1, <10 %; 2, 10–25 %; 3, 26–50 %; 4, 51–75 %; 5, >75 %. Ten random fields of view per kidney section (×200 magnification) were used for counting. b Number of necrotic tubules was measured in 10 random fields per kidney section. Data are the mean ± SE (n = 6/group); *p < 0.05 vs. I/R.c Periodic acid–Schiff (PAS) staining image from each group 

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