Fig. 1From: Acetylcholinesterase histochemistry (AChE) - A helpful technique in the diagnosis and in aiding the operative procedures of Hirschsprung diseaseFrozen section stained with rapid H&E shows (a) The presence of GC (arrows) (b) Hypertrophied nerve trunks (arrows) in the SM and no GC (c) Toluidin blue stain shows the presence of GC. (d) Normal nerve plexus shows positive ie black staining in the cytoplasm with negative staining of the nuclei of GC which stand out as negative shadows (arrow)Back to article page