Fig. 1

Overexpression of α-synuclein with wild type (wt) or mutant type (mt) reduces cytosolic HMGB1 and Beclin1 in PC12 cells. a and b: mRNA (a) and protein (b) levels of α-synuclein in the PC12 cell overexpressing wt α-synuclein, PC12 (Syn^wt), in the PC12 cell overexpressing mt (A53T and A30P) α-synuclein, PC12 (Syn^mt), in the PC12 cell overexpressing Enhanced Green Fluorescence Protein (EGFP), PC12 (Con), or in the blank P12 cells, the protein level of α-synuclein was examined by western blot analysis; c: Western blot analysis of HMGB1 (in cytosol and in nucleus) and Beclin1 (in cytosol) in PC12, PC12 (Con), PC12 (Syn^wt) or PC12 (Syn^mt) cells; d: Relative HMGB1 level to β-actin in cytosol or to Lamin B(LMNB1) in the nucleus of PC12, PC12 (Con), PC12 (Syn^wt) or PC12 (Syn^mt) cells; e: Relative Beclin1 level to β-actin in the cytosol of PC12, PC12 (Con), PC12 (Syn^wt) or PC12 (Syn^mt) cells; Each result was averaged for triple independent experiments. Statistical significance was presented as * p < 0.05, ** p < 0.01, *** p < 0.001, ns: no significance