Fig. 2
From: Beclin1 and HMGB1 ameliorate the α-synuclein-mediated autophagy inhibition in PC12 cells
Levels of autophagy-associated markers and autophagic vesicles induced by starvation in the PC12, PC12 (Con), PC12 (Synwt) or PC12 (Synmt) cells. a: Western blotting assay of LC3-I, LC3-II, Atg 7 and mTOR in the PC12, PC12 (Con), PC12 (Synwt) or PC12 (Synmt) cells post an incubation at 37 °C in FBS-free medium for 24 hours; b: Ratio of LC3-II to LC3-I in the starvation-treated cell lines; c: Relative level of Atg 7 and mTOR levels to β-actin in each cell line post the starvation treatment; d and e: Imaging (d) and counting (e) of EGFP-positive autophagic vesicles in the cytosol of starvation-treated PC12, PC12 (Con), PC12 (Synwt) or PC12 (Synmt) cells which were transfected with pCDNA3.1-EGFP-LC3 plasmid for another 24 hours; Data was averaged for triple independent results. * p < 0.05, ** p < 0.01, ns: no significance