Fig. 5
From: Beclin1 and HMGB1 ameliorate the α-synuclein-mediated autophagy inhibition in PC12 cells
Beclin1 upregulation reduces α-synuclein accumulation and ameliorates the α-synuclein-inhibited autophagy in PC12 (Synwt) and PC12 (Synmt) cells. a: Western blot analysis of Beclin1 and α-synuclein in the PC12 (Synwt) and PC12 (Synmt) cells which were infected with 1 multiplicity of infection (MOI) pLenti-Beclin1 (LV-Beclin1) or pLenti-Con (LV-Con) under starvation for 12 or 24 hours; b and c: Ratio of Beclin1 to β-actin in PC12 (Synwt) (b) and PC12 (Synmt) (c) cells which were infected with LV-Beclin1 or with LV-Con virus; d and e: Ratio of α-synuclein to β-actin in PC12 (Synwt) (b) and PC12 (Synmt) (c) cells which were infected with LV-Beclin1 or with LV-Con virus; f and g: Imaging (f) and counting (g) of EGFP-positive autophagic vesicles in the starvation-treated PC12 (Synwt) or PC12 (Synmt), which were infected with LV-Beclin1 or with LV-Con virus. Each result was averaged for triple independent experiments. Statistical significance was presented as * p < 0.05, ** p < 0.01, *** p < 0.001, ns: no significance