Table 1 The primary antibodies
Antibodies | Clone | Manufacturer | Dilution |
|---|---|---|---|
ER | 6F11 | Ventana, Tucson, AZ, USA | Ready to use |
PR | AB-52 | Santa Cruz Biotechnology, Dallas, Texas, USA | 1:100 |
HER2 | Polyclonal | Sigma-Aldrich Shanghai Trading Co Ltd Shanghai, China | Ready to use |
Ki67 | MIB-1 | DAKO, Denmark | Ready to use |
CK5/6 | D5/16B4 | DAKO, Denmark | 1:50 |
p63 | 4A4 | Fuzhou Maixin, Biology, Fouzhou, China | Ready to use |
CK14 | LL002 | Fuzhou Maixin, Biology, Fouzhou, China | Ready to use |
EGFR | Polyclonal | Santa Cruz Biotechnology, Dallas, Texas, USA | 1:100 |
EZH2 | D2C9 | Cell signaling Technology, Inc., Danvers, MA, USA | 1:100 |
H3K27me3 | C36B11 | Cell Signaling Technology, Inc., Danvers, MA, USA | 1:500 |