Table 1 Description of Anti-PD-L1 Antibodies and Assay Conditions Examined
From: Quantitative and qualitative characterization of Two PD-L1 clones: SP263 and E1L3N
Clone | Vendor | Species | Working Concentration | Instrument | Detection | Cell Conditioning | Diluent | Primary Antibody Time/Temp | Hematoxylin/Bluing |
|---|---|---|---|---|---|---|---|---|---|
SP263 | Spring | Rabbit | 1.25 μg/mL | Benchmark Ultra | OptiView | 64' CC1 | 0.05 M Tris-HCI with 1 % carrier protein, and 0.10 % ProClin 300, a preservative | 16'/36 °C | 4'/4' |
E1L3N | CST | Rabbit | 17.50 μg/mL | Benchmark Ultra | OptiView | 64' CC1 | 0.05 M Tris buffered saline, 0.01 M EDTA, 0.05 % Brij-35 with 0.3 % carrier protein and 0.05 % sodium azide, a preservative | 16'/36 °C | 4'/4' |