Skip to main content

Table 1 Description of Anti-PD-L1 Antibodies and Assay Conditions Examined

From: Quantitative and qualitative characterization of Two PD-L1 clones: SP263 and E1L3N

Clone Vendor Species Working Concentration Instrument Detection Cell Conditioning Diluent Primary Antibody Time/Temp Hematoxylin/Bluing
SP263 Spring Rabbit 1.25 μg/mL Benchmark Ultra OptiView 64' CC1 0.05 M Tris-HCI with 1 % carrier protein, and 0.10 % ProClin 300, a preservative 16'/36 °C 4'/4'
E1L3N CST Rabbit 17.50 μg/mL Benchmark Ultra OptiView 64' CC1 0.05 M Tris buffered saline, 0.01 M EDTA, 0.05 % Brij-35 with 0.3 % carrier protein and 0.05 % sodium azide, a preservative 16'/36 °C 4'/4'