Fig. 3

Immunocytochemical analysis of HIF-1α in LNCaP and PC-3 cells, under normoxia and hypoxia. No staining or mild cytoplasmic positivity for HIF-1α is detected in LNCaP (a) and PC-3 (b) cells, under normoxia. Under hypoxia, cytoplasmic positivity becomes stronger, both in LNCaP (c) and PC-3 cells (d). Nuclear translocation of the protein is also evident (c-d) (a-d, O.M.: 400x)