Quantitative comparison and reproducibility of pathologist scoring and digital image analysis of estrogen receptor β2 immunohistochemistry in prostate cancer

Table 4 Hazard ratios (HRs) of PCa recurrent free survival and PCa-specific mortality after radical prostatectomy by ERβ2 staining in tumor epithelium quantified by image analysis (per tertile increment)

	RFS		PCSM
	HR (95 % CI)	p-value	HR (95 % CI)	p-value
Cytoplasmic Digital IHC (CytoOD*%PosCyto)
Univariate	1.07 (0.85, 1.34)	0.561	2.16 (1.02, 4.57)	0.045
Multivariate ^a	1.06 (0.84, 1.33)	0.624	1.98 (0.93, 4.21)	0.075
Nuclear Digital IHC (NucOD*%PosNuc)
Univariate	1.11 (0.89, 1.40)	0.352	2.67 (1.20, 5.96)	0.016
Multivariate ^a	1.00 (0.79, 1.27)	0.999	2.32 (0.99, 5.41)	0.052
Total Malignant Epithelial Area Digital IHC (OD*%Pos)
Univariate	1.25 (0.99, 1.57)	0.057	5.10 (1.70, 15.34)	0.004
Multivariate ^a	1.19 (0.94, 1.51)	0.150	4.08 (1.37, 12.15)	0.012

^a Adjusted for age at diagnosis (years), Gleason score (≤6, 7[3 + 4], 7[4 + 3], and ≥8), pathological stage (local: pT2, N0/NX, M0; regional: pT3/pT4 or N1-3, M0), and diagnostic PSA level (1 unit increase)