Fig. 5From: MiR-424-5p participates in esophageal squamous cell carcinoma invasion and metastasis via SMAD7 pathway mediated EMT a b Real-time PCR and western blot analysis showed that the SMAD7 mRNA and protein expression in EC-1 cells transfected with pcDNA3.1-SMAD7 increased greatly compared to blank EC-1 cells. ☆ < 0.05 vs blank control. Furthermore, pcDNA3.1-SMAD7 transfection could partially enhance the EMT decreased by miR-424-5p mimics transfection by increasing the mRNA and protein expression of E-cadherin, while decreasing the mRNA and protein expression of vimentin. *p < 0.05 vs miR-424-5p mimicsBack to article page