Table 2 The results of FISH and IHC method to evaluate three samples made of different cell lines for quality control
Quality control samples | Cell lines’ name or patient’s code | The results of FISH detection | The results of IHC detection | |||
|---|---|---|---|---|---|---|
Qualitative results | Ratio of HER2:chromosome 17 | HER2 copy number | Qualitative results | IHC scoring | ||
Cell lines samples | MCF-7 | Negative | 1.15 | 3.05 | Negative | 0 |
MDA-MB-453 | Positive | 2.26 | 6.9 | Positive | 3+ | |
SKBR-3 | Positive | Clusters | Clusters | Positive | 3+ | |
BT474 | Positive | Clusters | Clusters | Positive | 3+ | |
Agarose gel samples | MCF-7 | Negative | 1.18 | 3.85 | Negative | 0 |
MDA-MB-453 | Positive | 2.15 | 7.1 | Negative | 0 | |
SKBR-3 | Positive | Clusters | Clusters | Negative | 1+ | |
BT474 | Positive | Clusters | Clusters | Positive | 2+ | |
Xenograft tumor samples | MCF-7 | Negative | 1.27 | 5.25 | Negative | 0 |
MDA-MB-453 | Positive | 2.42 | 8 | Positive | 2+ | |
SKBR-3 | Positive | Clusters | Clusters | Positive | 3+ | |
BT474 | Positive | Clusters | Clusters | Positive | 3+ | |
Clinical specimens | A2 | Negative | 1.07 | 4.07 | Negative | 0 |
A12 | Positive | Clusters | Clusters | Positive | 3+ | |