TY - JOUR AU - Akhter, Ariz AU - Mughal, Muhammad Kashif AU - Elyamany, Ghaleb AU - Sinclair, Gary AU - Azma, Raja Zahratul AU - Masir, Noraidah AU - Shuib, Salwati AU - Rashid-Kolvear, Fariborz AU - Shabani-Rad, Meer-Taher AU - Stewart, Douglas Allan AU - Mansoor, Adnan PY - 2016 DA - 2016/09/15 TI - Multiplexed automated digital quantification of fusion transcripts: comparative study with fluorescent in-situ hybridization (FISH) technique in acute leukemia patients JO - Diagnostic Pathology SP - 89 VL - 11 IS - 1 AB - The World Health Organization (WHO) classification system defines recurrent chromosomal translocations as the sole diagnostic and prognostic criteria for acute leukemia (AL). These fusion transcripts are pivotal in the pathogenesis of AL. Clinical laboratories universally employ conventional karyotype/FISH to detect these chromosomal translocations, which is complex, labour intensive and lacks multiplexing capacity. Hence, it is imperative to explore and evaluate some newer automated, cost-efficient multiplexed technologies to accommodate the expanding genetic landscape in AL. SN - 1746-1596 UR - https://doi.org/10.1186/s13000-016-0541-z DO - 10.1186/s13000-016-0541-z ID - Akhter2016 ER -