Fig. 3

Immunohistochemical patterns of the tumor. a–b Neoplastic epithelial cells with strong nuclear and cytoplasmic staining for MMP-3 and MMP-9 (immunostaining, original magnification × 20). c Variable collagen IV reactivity around neoplastic epithelial proliferations (immunostaining, original magnification × 20). d Neoplastic epithelial cells from tumor advancing edge with strong membrane and cytoplasmic staining for Beta-catenin (immunostaining, original magnification × 20), e Neoplastic epithelial cells from tumor advancing edge with strong membrane and nuclear staining for N-cadherin (immunostaining, original magnification × 20). f Neoplastic epithelial cells from tumor advancing edge with E-cadherin (brown staining) and vimentin (red staining) cytoplasmic colocalization (double immunostaining, original magnification × 20). g Neoplastic epithelial cells from tumor advancing edge with strong cytoplasmic and nuclear reactivity for Slug 1 (immunostaining, original magnification × 20). h Neoplastic epithelial cells with strong membrane, cytoplasmic and nuclear reactivity for CXCR4 (double immunohistochemical detection CXCR4-brown staining and vimentin- red staining) (double immunostaining, original magnification × 20). i Podoplanin tumor cell reactivity, with a membranous pattern at the advancing edge, mainly at the periphery of tumor proliferations (immunostaining, original magnification × 10)