Table 3 Summary of primary antibody used for immunohistochemistry
Antibody | Isotype | Supplier | Dilution |
|---|---|---|---|
EpCAM | IgG1(rabbit) | Epitomics | 1:300 |
EpCAM | IgG1(mouse) | abcam | 1:100 |
Cytokeratin 7 | IgG1(mouse) | DAKO | 1:100 |
E-Cadherin | IgG1(mouse) | DAKO | 1:50 |
S100A4 | IgG(rabbit) | abcam | 1:50 |
MMP-2 | IgG(rabbit) | abcam | 1:250 |
Twist | IgG1(mouse) | abcam | 1:150 |
Snail | IgG(rabbit) | abcam | 1:100 |
Vimentin | IgG1(mouse) | DAKO | 1:100 |
α-SMA | IgG2a(mouse) | DAKO | 1:100 |