Table 4 The sequences of primers used in qRT-PCR
Gene | Sense | Antisense | Product (bp) |
|---|---|---|---|
EpCAM | CTGGACTGGAATGCTGAG | CGAAGATGACGATGAGGAT | 178 |
S100A4 | GATGAGCAACTTGGACAGCAA | CTGGGCTGCTTATCTGGGAAG | 123 |
MMP-2 | CCGTCGCCCATCATCAAGTT | CTGTCTGGGGCAGTCCAAAG | 169 |
Twist | GAATGACCGCTTCGCCAACTA | CCGCATCTCCTCCTCGTAGA | 136 |
Snail | ACTACTGCTGAGCGTGAGATTG | CGATGAAGGATGGCTGGAACA | 199 |
CK7 | TTGTGGTGCTGAAGAAGG | CTGTCAACTCCGTCTCATT | 123 |
E-cadherin | CGAGAGCTACACGTTCACGG | GTGTCGAGGGAAAAATAGGCTG | 117 |
Vimentin | GACGCCATCAACACCGAGTT | CTTTGTCGTTGGTTAGCTGGT | 238 |
αSMA | GTGTTGCCCCTGAAGAGCAT | GCTGGGACATTGAAAGTCTCA | 109 |
GAPDH | GAAGATGGTGATGGGATTTC | GAAGGTGAAGGTCGGAGT | 230 |