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Table 7 Comparison of methodology used for FFPE muscle in the present and other studies

From: Immunohistochemistry of sarcolemmal membrane-associated proteins in formalin-fixed and paraffin-embedded skeletal muscle tissue: a promising tool for the diagnostic evaluation of common muscular dystrophies

Studies Pretreatment buffer (duration) Primary antibody incubation time Thickness of FFPE sections (μm)
Present study Citrate/EDTA (40 min) Overnight 3 and 5
Hoshino et al. 2000 [17] Target retrieval solution (20 min) 15 min 6
Sheriffs et al. 2001 [18] Citrate/EDTA (4 min) Overnight 8
Sajid et al. 2010 [19] Citrate/EDTA (not stated) 2 h 8