Table 7 Comparison of methodology used for FFPE muscle in the present and other studies
Studies | Pretreatment buffer (duration) | Primary antibody incubation time | Thickness of FFPE sections (μm) |
|---|---|---|---|
Present study | Citrate/EDTA (40 min) | Overnight | 3 and 5 |
Hoshino et al. 2000 [17] | Target retrieval solution (20 min) | 15 min | 6 |
Sheriffs et al. 2001 [18] | Citrate/EDTA (4 min) | Overnight | 8 |
Sajid et al. 2010 [19] | Citrate/EDTA (not stated) | 2 h | 8 |