Fig. 1
From: Detection of 22 common leukemic fusion genes using a single-step multiplex qRT-PCR-based assay
Molecular and cytogenetic screening in hematological malignancies. a Cytogenetic analysis of bone marrow biopsy identified with a karyotypic chromosomal rearrangement inv(16)(p13;q22) (black arrow). b PCR fluorescent chart showing amplification curve for both targeted gene fusion and housekeeping reference cDNA products. c DNA sequence (top) and chromatograph (bottom) of a CBFβ-MYH11 fusion transcript (cDNA). Chromosomal breakpoint is indicated by a red line within the DNA sequence. Contributing exons from each gene (DBFB exon 5 and MYH11 exon 7) are indicated below (blue and red shaded labeled regions)