Table 3 PCR reaction tube system
From: Detection of 22 common leukemic fusion genes using a single-step multiplex qRT-PCR-based assay
PCR tube | Target gene | Fluorescent Channel |
|---|---|---|
PCR tube 1 | BCR-ABL | FAM |
SIL-TAL1 | HEX | |
E2A-HLF | CY5 | |
PCR tube 2 | TEL-AML1 | FAM |
MLL-AF4 | HEX | |
E2A-PBX1 | CY5 | |
PCR tube 3 | RUNX1-RUNX1T1 | FAM |
MLL-AF9 | HEX | |
PML-RARα | CY5 | |
PCR tube 4 | MLL-AF6, −AF10, −ELL, −ENL | HEX |
PLZF-RARα,STAT5b-RARα | FAM | |
NPM-MLF | CY5 | |
PCR tube 5 | TEL-PDGFRB | FAM |
FIP1L1-PDGFRA | HEX | |
AML1-MDS1/EVI1,AML1-MTG16 | CY5 | |
PCR tube 6 | CBFβ-MYH11 | FAM |
DEK-CAN | HEX | |
ABL (reference gene) | CY5 |