Table 4 Comparison of diagnostic results obtained by cytogenetic analysis and multi-fusion gene F-qRT-PCR screening
From: Detection of 22 common leukemic fusion genes using a single-step multiplex qRT-PCR-based assay
Subtype | Cytogenetics | F-qRT-PCR | ||||||
|---|---|---|---|---|---|---|---|---|
Cyto- | Cyto+ | % (+) | Translocation | PCR- | PCR+ | % (+) | Fusion gene | |
M1 | 4 | Â | 0% | none | 4 | Â | 0% | None |
AML M2 | 1 | Â | Â | none | 1 | Â | Â | None |
a 1 | Â | 90.5% | none | Â | 1 | 95.2% | RUNX1-RUNX1T1 | |
| Â | 17 | t(8;21)(q22;q22) | Â | 17 | RUNX1-RUNX1T1 | |||
| Â | 1 | t(16;21)(q24;q22) | Â | 1 | AML1-MDS/EVII/MTG16 | |||
| Â | 1 | del(4)(q12) | Â | 1 | FIP1L1-PDGFRA | |||
AML M3 | 3 | Â | Â | none | 3 | Â | Â | None |
|  | 43 | 93.5% | t(15;17)(q22;q12) |  | 43 | 93.5% | PML-RARα | |
AML M4 | 2 | Â | Â | none | 2 | Â | Â | None |
| Â | 1 | 33.3% | t(8;21)(q22;q22) | Â | 1 | 33.3% | RUNX1-RUNX1T1 | |
AML M5 | 22 | Â | Â | none | 22 | Â | Â | None |
|  | 2 | 12.0% | inv(16)(p13;q22) |  | 2 | 12.0% | CBFβ-MYH11 | |
| Â | 1 | t(9;11)(p22;q23) | Â | 1 | MLL-AF9 | |||
AML M6 | 1 | Â | Â | none | 1 | Â | Â | None |
| Â | 1 | 50.0% | del(1p) | Â | 1 | 50.0% | SIL-TAL1 | |
Undecided |  | 3 | 100.0% | inv(16)(p13;q22) |  | 3 | 100.0% | CBFβ-MYH11 |
| Â | 3 | t(6;11)(q26;q23) | Â | 3 | MLL-AF6/AF10/ELL/ENL | |||
| Â | 1 | del(1p) | Â | 1 | SIL-TAL1 | |||
subtotal | 34 | 74 | 68.5% | Â | 33 | 75 | 69.4% | Â |