Variables | Definition | Category | Explanation of category |
---|---|---|---|
Papillary fusion | Fusion of papillae with forming confluent and complex papillary cores | 1 | <1/3 area |
2 | 1/3Â ~Â 2/3 area | ||
3 | >2/3 area | ||
Umbrella cells | Area with preserved umbrella cells | 1 | >50% area |
2 | 5-50% area | ||
3 | <5% area | ||
Discohesiveness | Detached cells from papillae | 1 | <1/3 area |
Refer to Additional file 1: Figure S1. | 2 | 1/3Â ~Â 2/3 area | |
3 | >2/3 area | ||
Cell density | Â | 1 | <1/3 areas show more than 2 times normal density |
2 | 1/3 ~ ≤2/3 area shows more than 2 times of normal density | ||
3 | >2/3 area show more than 2 times normal density or >5% area shows more than 3 times normal density | ||
Nuclear pleomorphism | Size of smallest nuclei vs. largest nuclei(regardless of tangential sectioning) | 1 | <3 |
2 | 3 ≤ and <8 | ||
3 | 8 ≤ and <20 | ||
4 | ≥20 | ||
Multinucleated giant cells | Presence of bi-or multinucleated nuclei | 0 | Absence |
1 | Presence | ||
Loss of polarity | Proportion of cells deviating from the vertical alignment shown in normal urothelium | 1 | <5% |
2 | 5-50% | ||
3 | >50% | ||
Hyperchromasia | Semiquantitative degree of nuclear hyperchromasia | 1 | diffusely mild |
2 | diffusely moderate or focally strong | ||
3 | diffusely strong | ||
Nuclear groove | Proportion of tumor cells without identifiable nuclear grooves | 1 | <5% |
2 | 5-50% | ||
3 | > 50% | ||
Prominent nucleoli | Proportion of cells having prominent nuclei (recognizable under 10× medium power) | 1 | <5% |
2 | 5-50% | ||
3 | >50% | ||
Whorling pattern | Refer to Additional file 1: Figure S1. | 0 | absent |
1 | present | ||
Necrosis | Degree of necrosis | 1 | singly spotted |
2 | focally grouped or multifocally spotted | ||
3 | surface necrosis of nests | ||
4 | confluent necrosis | ||
Divergent histology | Presence and number of glandular, squamous or micropapillary differentiation | 0 | absence |
1 | 1 | ||
2 | 2 | ||
3 | 3 | ||
Mitotic count | Number of mitosis/ 10 consecutive HPFs in most mitotically active area | CON | Â |
Mitotic count-CAT | Group of mitotic count | 1 | 0-2 |
2 | 3-7 | ||
3 | 8-15 | ||
4 | ≥16 | ||
Mitotic level | Most highest level of mitotic figures, from base to the top of the papilla(low 1/3, mid1/3, and high 1/3) | 1 | <1/3 or no mitosis |
2 | 1/3-2/3 | ||
3 | >2/3 | ||
Apoptosis | Number of apoptotic bodies in most active area/one HPF | 1 | <10 |
2 | 10-100 | ||
3 | >100 | ||
Capillary proliferation | Number of capillary in one papillary core | CON | Â |