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Fig. 4 | Diagnostic Pathology

Fig. 4

From: Computer-assisted stereology and automated image analysis for quantification of tumor infiltrating lymphocytes in colon cancer

Fig. 4

Field of vision in a CD3-stained section magnified ×40. The density estimation was performed using the 2D unbiased counting frame with left and bottom edges, and their extensions, serving as exclusion lines (red), and with the upper and right edges of the frame as inclusion lines (green). Cell profiles were counted when completely inside the counting frame or partly inside the frame, provided that they did not touch the exclusion lines or their extensions. Thus, three cell profiles were counted (red crosses). The area fraction estimation was performed using the point grid. Points hitting CD3 positive cells = 2 (red ring) and points hitting tumor = 30, giving an area fraction of 0.07 in this field of vision

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