Computer-assisted stereology and automated image analysis for quantification of tumor infiltrating lymphocytes in colon cancer

Table 5 Correlation between numerical density and area fraction

	Spearman correlation All sections (n = 129)	Spearman correlation Deepest section (n = 43)	Spearman correlation Random section A (n = 43)	Spearman correlation Random section B (n = 43)
CD3-Central Stereology	0.9575	0.9503	0.9688	0.9448
CD3-Central Image analysis	0.9926	0.9872	0.9878	0.9899
CD8-Central Stereology	0.9796	0.9838	0.9578	0.9872
CD8-Central Image analysis	0.9932	0.9869	0.9941	0.9917
CD3-Invasive Stereology	0.9519	0.9457	0.9443	0.9438
CD3-Invasive Image analysis	0.9890	0.9905	0.9787	0.9962
CD8-Invasive Stereology	0.9676	0.9665	0.9713	0.9565
CD8-Invasive Image analysis	0.9907	0.9917	0.9912	0.9878

Correlation between numerical densities and area fraction obtained by either stereology or image analysis for all tumor sections and for individual sections. Spearman’s correlation, p < 0.0001

ISSN: 1746-1596