Table 1 Antibodies used for immunohistochemical staining
From: Splenic hamartoma with bizarre stromal cells: a case report and literature review
Antibody | Major Specificity | Source | Dilution |
|---|---|---|---|
Epithelial marker | |||
Cytokeratin (AE1/AE3) | Pan-cytokeratin | Novocastra | 1:100 |
Mesenchymally-derived cell marker | |||
Vimentin (V9) | Mesenchymally-derived cell | Dako | 1:1000 |
Endothelial markers | |||
CD31 (JC/70A) | Endothelial cells, megakaryocytes, plasma cells | Dako | 1: 50 |
CD34 (QBEnd/10) | Endothelial cells, some stromal cells | Dako | 1:200 |
Fli-1 (G146-222) | Endothelial cells, Ewing’s sarcoma | Maixin | RTU |
Lymphoid and myeloid markers | |||
CD3 (LN10) | T cells | Novocastra | 1:100 |
CD20 (L26) | B cells | Novocastra | 1:250 |
CD8 (1A5) | Cytotoxic T cells, splenic sinus-lining cells | Novocastra | 1: 50 |
CD15 (Carb-3) | Reed-Sternberg cells, granulocytes, histiocytes | Dako | RTU |
CD30 (Ber-H2) | Activated lymphoid cells, Reed-Sternberg cells, anaplastic large cell lymphoma | Dako | 1:40 |
ALK (5A4) | Anaplastic large cell lymphoma, HL | Novocastra | RTU |
Pax-5 (SP34) | B cells | Ventana | RTU |
CD61 (2f2) | Megakaryocyte | Novocastra | 1:100 |
MPO (59A5) | Myeloid cells | Novocastra | 1:1000 |
Histiocyte and monocyte marker | |||
CD68 (514H12) | Histiocytes and monocytes | Novocastra | 1:100 |
Follicular dendritic cell markers | |||
CD21 (2G9) | Follicular dendritic cells | Novocastra | RTU |
CD35 (RLB25) | Follicular dendritic cells | Novocastra | 1:50 |
Myogenic markers | |||
Smooth muscle actin (αsm-1) | Muscle cells, myofibroblasts, pericytes | Novocastra | 1:200 |
Desmin (DE-R-11) | Muscle cells, myofibroblasts | Novocastra | 1:200 |
Langerhans cell markers | |||
S-100 protein (antiserum) | Melanocytes, dendritic cells, nerve sheath cells, cartilage cells myoepithelial cells, fat cells | Dako | 1:1000 |
CD1α (010) | T cells, Langerhans cells, thymocyte | Dako | 1:50 |
Proliferation-related marker | |||
Ki-67 (MIB-1) | Cell proliferation activity | Dako | 1:100 |