Fig. 2From: Physical basis of the ‘magnification rule’ for standardized Immunohistochemical scoring of HER2 in breast and gastric cancerWidth and color intensity of HER2 are correlated. Immunohistochemistry of n = 120 cases of invasive ductal carcinoma of the mammary gland show that width of the linear DAB precipitates (a) and DAB color intensity (b) are correlated (8bit scale, inverted relative values). However, neither parameter discriminates IHC Score 2+ samples that are Her2 amplified (ISH+) or not amplified (ISH-) by in situ hybridizationBack to article page