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Table 1 Primary and secondary antibodies for the FICTION method

From: The t(14;18) translocation is absent from endothelial and follicular dendritic cells of follicular lymphoma (FL) and shows heterogeneous presence in preserved FL mantle zones

Primary Ab Source Dilution Incubation Time Secondary Ab Source Dilution Incubation Time Conjugated with
Polyclonal Ab Rabbit anti-Human IgD
Code: A0093
DAKO/Agilent Technolo. 1:400 60 min/RT Goat anti Rabbit IgG (H + L) Jackson ImmunoResearch (Dianova distrib.) 1:25 60 min/RT Cy™3, Code:111–165-144
mAb Mouse anti-Human CD34 Class II Clone QBEnd-10
Code: M7165
DAKO/Agilent Technolo. 1:50 60 min/RT Goat anti Mouse IgG (H + L) Jackson ImmunoResearch (Dianova distrib.) 1:50
1:25
1:50
60 min/RT Cy™2, Code:115–228-003
Cy™3, Code:115–165-003
Alexa Flourʘ 488, Code: 115–545-003
mAb Mouse anti-Human IgG1 kappa Clusterin (Apolipoprotein J)
Code: NCL-CLUSTERIN
Novocastra (LEICA distribut.) 1:30 60 min/RT Goat anti Mouse IgG (H + L) Jackson ImmunoResearch (Dianova distrib.) 1:50
1:25
1:50
60 min/RT Cy™2, Code:115–228-003
Cy™3, Code:115–165-003
Alexa Flourʘ 488, Code: 115–545-003