Table 1 Primary and secondary antibodies for the FICTION method
Primary Ab | Source | Dilution | Incubation Time | Secondary Ab | Source | Dilution | Incubation Time | Conjugated with |
|---|---|---|---|---|---|---|---|---|
Polyclonal Ab Rabbit anti-Human IgD Code: A0093 | DAKO/Agilent Technolo. | 1:400 | 60 min/RT | Goat anti Rabbit IgG (H + L) | Jackson ImmunoResearch (Dianova distrib.) | 1:25 | 60 min/RT | Cy™3, Code:111–165-144 |
mAb Mouse anti-Human CD34 Class II Clone QBEnd-10 Code: M7165 | DAKO/Agilent Technolo. | 1:50 | 60 min/RT | Goat anti Mouse IgG (H + L) | Jackson ImmunoResearch (Dianova distrib.) | 1:50 1:25 1:50 | 60 min/RT | Cy™2, Code:115–228-003 Cy™3, Code:115–165-003 Alexa Flourʘ 488, Code: 115–545-003 |
mAb Mouse anti-Human IgG1 kappa Clusterin (Apolipoprotein J) Code: NCL-CLUSTERIN | Novocastra (LEICA distribut.) | 1:30 | 60 min/RT | Goat anti Mouse IgG (H + L) | Jackson ImmunoResearch (Dianova distrib.) | 1:50 1:25 1:50 | 60 min/RT | Cy™2, Code:115–228-003 Cy™3, Code:115–165-003 Alexa Flourʘ 488, Code: 115–545-003 |