Table 3 Primary antibodies and their conditioning in this study
Antibody | Clone | Source | Dilution | Conditioning |
|---|---|---|---|---|
TLE1 | EPR9386 (2) | Abcam, Cambridge, UK | 1:100 | pH 9.0 |
β-catenin | 17C2 | Novocastra, Newcastle, UK | 1:100 | pH 6.0 overnight |
Ki67 | MIB-1 | DAKO, Santa Clara, USA | 1:50 | pH 6.0 |
αSMA | 1A4 | DAKO, Santa Clara, USA | 1:100 | |
EMA | E29 | Nichirei Bioscience, Tokyo, Japan | Diluted |