Fig. 4

Characterization of the HPV18 L1-VLP Detection Kit developed in this study. a The linearity of the HPV18 L1-VLP Detection Kit. Each well was coated with 10 μg/ml of mAb 4C2, blocked with 3% BSA-PBST, and air-dried. Different concentrations (0.1, 0.3, 1.3, and 10 μg/ml) of the HPV18 L1-VLP standards were diluted in 3% BSA-PBST and tested in triplicate. The positive control was defined as being the mean of greater than 2.1times the negative control. The standard curve was sigmoidally fitted with 4 parameters; x axis is the logarithm of HPV18 L1-VLP concentrations; y axis was the optical density at 450 nm (OD₄₅₀); b The specificities for native and denatured HPV L1-VLPs of the Detection Kit. The OD₄₅₀ values of all samples tested were plotted against various genotypes of HPV L1-VLPs (both native and denatured). The bar graph was prepared using Prism GraphPad 6.0. All L1-VLPs samples were adjusted to an initial concentration of 30 μg/ml (100 μl per well). All of the values were the average of multiple samples