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Fig. 5 | Diagnostic Pathology

Fig. 5

From: PD1hi cells associate with clusters of proliferating B-cells in marginal zone lymphoma

Fig. 5

PD1hi cells cluster with Ki-67+ cells. a Illustrative cartoon depicting low and high interactions with the respective Pearson correlation and Morisita-Horn index for both patterns. b Spatial interaction between FOXP3 or PD1 and Ki-67 cells. Scatter plots displaying Ki67+ against PD1hi cells (upper panel) and FOXP3+ cells (lower panel) measured in spatial quadrants (200μm2). Only quadrants containing at least 5 cells of any population are displayed. Quadrants containing higher densities of both cell types (> 50 cells) were considered to represent high interaction. 11.3% of all quadrants analysed for Ki67+ and PD1hi cells met this definition of high interaction compared to only 1.6% of Ki67+ and FOXP3+ cells. Analysis was repeated for different sized quadrants (100 and 400μm2) confirming similar results. c Nearest neighbour analysis. Distance from either FOXP3+ cells (upper panel) or PD1hi cells (lower panel) was plotted for 105 cells sampled from 10 cases. For PD1hi cells the large peak occurring at short distances to the nearest Ki-67+ cell indicates that these cells are closer to Ki-67+ cells than FOXP3+ cells, which are more evenly distributed. All clustering and spatial analysis was performed using the statistical programme R

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