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Table 2 Antibodies used in this study and staining conditions

From: Expression of HMB45, MelanA and SOX10 is rare in non-small cell lung cancer

Antibody Company Clone Pretreatment Buffer incubation time (min) Antibody incubation time (min) Dilution
CK5/6 Dako D5/16 B4 Tris/Borat/EDTA, pH 9 56 24 1:50
HMB45 Dako HMB45 Tris/Borat/EDTA, pH 8.4 64 24 1:75
MelanA Ventana A103 Tris/Borat/EDTA, pH 8.4 64 24 RTU
SOX10 Cell Marque EP268 Tris/Borat/EDTA, pH 8.4 48 24 1:100
Napsin A Novocastra IP64 Tris/Borat/EDTA, pH 6 32 24 1:400
p63 DCS Immunoline 4A4 Tris/Borat/EDTA, pH 6 40 24 1:25
TTF-1 Novocastra SPT24 Tris/Borat/EDTA, pH 9 56 24 1:200
  1. CK, cytokeratin, HMB45, Human Melanoma Black 45, SOX10, SRY-related HMG-box 10 protein, TTF-1, thyroid transcription factor-1