Table 2 Antibodies used in this study and staining conditions
From: Expression of HMB45, MelanA and SOX10 is rare in non-small cell lung cancer
Antibody | Company | Clone | Pretreatment | Buffer incubation time (min) | Antibody incubation time (min) | Dilution |
|---|---|---|---|---|---|---|
CK5/6 | Dako | D5/16 B4 | Tris/Borat/EDTA, pH 9 | 56 | 24 | 1:50 |
HMB45 | Dako | HMB45 | Tris/Borat/EDTA, pH 8.4 | 64 | 24 | 1:75 |
MelanA | Ventana | A103 | Tris/Borat/EDTA, pH 8.4 | 64 | 24 | RTU |
SOX10 | Cell Marque | EP268 | Tris/Borat/EDTA, pH 8.4 | 48 | 24 | 1:100 |
Napsin A | Novocastra | IP64 | Tris/Borat/EDTA, pH 6 | 32 | 24 | 1:400 |
p63 | DCS Immunoline | 4A4 | Tris/Borat/EDTA, pH 6 | 40 | 24 | 1:25 |
TTF-1 | Novocastra | SPT24 | Tris/Borat/EDTA, pH 9 | 56 | 24 | 1:200 |