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Fig. 2 | Diagnostic Pathology

Fig. 2

From: Widely metastatic IDH1-mutant glioblastoma with oligodendroglial features and atypical molecular findings: a case report and review of current challenges in molecular diagnostics

Fig. 2

Routine hematoxylin and eosin stains (H&E) showed infiltrative sheets of discohesive neoplastic cells (a, 200X; inset, 400X). The majority of cells demonstrated high nuclear to cytoplasmic ratios with round hyperchromatic nuclei and moderate nuclear pleomorphism. A subset of cells showed gemistocytic morphology with eccentrically displaced dense eosinophilic cytoplasm (a, 200X; inset, 400X). Occasional giant cells were also noted. Ancillary immunohistochemical stains showed that the neoplasm contained some areas of IDH1-mutant protein positive cells and other areas that were predominantly IDH1-mutant protein negative to weakly positive (d, 200X; inset, 200X). GFAP (c, 200X; inset, 200X) and OLIG2 (b, 200X; inset, 200X) expression was also variable. ATRX protein expression was uniformly retained throughout (e, 200X) and synaptophysin was diffusely positive (f, 200X). The neoplasm also co-expressed NeuN and S-100 protein, while chromogranin was focal (not shown). INI-1 and BRG1 were retained. Additional stains for CD99, HH3 K27 M, SOX10, CD30, myogenin, desmin, CAM5.2 (CK8/18), AE1/AE3, OCT3/4, Melan A, CD45, CD3, and CD20 were negative in the tumor

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Diagnostic Pathology

ISSN: 1746-1596

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