Skip to main content
Fig. 3 | Diagnostic Pathology

Fig. 3

From: Association of tumour-associated macrophages with cancer cell EMT, invasion, and metastasis of Kazakh oesophageal squamous cell cancer

Fig. 3

TAMs induce the cancer cells to undergo EMT. (a) PMA-treated THP-1 cells were differentiated into adherent macrophages. (b) EC109 and EC9706 cells presented typical cobblestone and epithelial-like appearances, respectively; with CM treatment, they were scattered and spindle shaped, and exhibited fibroblast-like appearances. The photographs were taken at 100x magnification. (c) The relative mRNA expression levels of E-cadherin and vimentin were determined by qPCR, respectively. The housekeeping gene GAPDH was used as the control. (d) The EMT-related proteins E-cadherin and vimentin were examined by western blotting, respectively; β-actin was used as a loading control. (e) and (f) Macrophages promote the migration and invasion of ESCC cells, respectively. * P < 0.05, ** P < 0.01, *** P < 0.001

Back to article page