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Fig. 2 | Diagnostic Pathology

Fig. 2

From: LncRNA DNM3OS promotes proliferation and inhibits apoptosis through modulating IGF1 expression by sponging MiR-126 in CHON-001 cells

Fig. 2

Over-expression of DNM3OS enhanced proliferation and suppressed apoptosis in CHON-001 cells, while DNM3OS silencing had opposite effects. (a) CHON-001 cells proliferation was improved by DNM3OS, while silencing DNM3OS inhibited CHON-001 cells proliferation as indicated by cloning formation experiment. (b) The number of proliferated cells was quantified. (c) CHON-001 cells apoptosis was inhibited by DNM3OS, while DNM3OS silencing induced apoptosis as indicated by flow cytometry. (d) The proportion of apoptosis was quantified. (e) The apoptosis-related proteins (Bcl-2, Bax and c caspase 3) were detected by Western blot. (f) The relative levels of proteins described in (e) were counted by GAPDH as normalization. aP < 0.05 vs. Blank, bP < 0.05 vs. NC, cP < 0.05 vs. DNM3OS, dP < 0.05 vs. siNC. siDNM3OS: small interfering dynamin 3 opposite strand; NC: negative control; Bcl-2: B-cell lymphoma-2; Bax: Bcl2-associated X protein

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