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Fig. 5 | Diagnostic Pathology

Fig. 5

From: LncRNA DNM3OS promotes proliferation and inhibits apoptosis through modulating IGF1 expression by sponging MiR-126 in CHON-001 cells

Fig. 5

The down-regulation of miR-126 abolished the DNM3OS knockdown-mediated inhibition of cells proliferation and promotion of cell apoptosis. (a) Co-transfecting si-DNM3OS and miR-126 inhibitor promoted cell proliferation as shown by cloning formation experiment. (b) The number of proliferated cells was quantified. (c) Cells apoptosis was triggered by silencing DNM3OS, which partially reversed by miR-126 inhibitor. (d) The proportion of apoptosis was quantified. (e) The apoptosis-related proteins (Bcl-2, Bax and c caspase 3) were detected by Western blot. (f) The relative levels of proteins described in (e) were counted by GAPDH as normalization. aP < 0.05 vs. MC, bP < 0.05 vs. M, cP < 0.05 vs. IC, dP < 0.05 vs. I, eP < 0.05 vs. siDNM3OS. M: miR-126 mimic; I: miR-126 inhibitor; MC: miR-126 mimic control; IC: miR-126 inhibitor control; siDNM3OS: small interfering dynamin 3 opposite strand; Bcl-2: B-cell lymphoma-2; Bax: Bcl2-associated X protein

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Diagnostic Pathology

ISSN: 1746-1596

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