Fig. 7

MiR-126 directly targeted IGF1 in CHON-001 cells. (a) The predicted binding sequences of IGF1 in miR-126 using targetscan 7.2. (b) The luciferase intensity of cells transfected with miR-126 wild-type and IGF1 was significantly reduced. (c) The protein level of IGF1 was decreased in miR-126 mimic-treated cells. (d) The mRNA and protein level of IGF1 were quantified. (e) CHON-001 cells viability was higher in mimic+IGF1 than that in mimic group. ^aP < 0.05 vs. IGF1-WT or MC + NC, ^bP < 0.05 vs. MC + IGF1, ^cP < 0.05 vs. mimic+NC. Mimic: miR-126 mimic; MC: miR-126 mimic control; WT: wild-type; MUT: mutated-type