Fig. 8

Restored IGF1 expression improved the suppressive effects of miR-126 overexpression in cell proliferation and attenuated the promotion effects of miR-126 overexpression in cell apoptosis. (a) Co-transfecting miR-126 mimic and IGF1 promoted cell proliferation as shown by cloning formation experiment. (b) The number of proliferated cells was quantified. (c) Cells apoptosis was triggered by miR-126 mimic, which partially reversed by IGF1. (d) The proportion of apoptosis was quantified. (e) The apoptosis-related proteins (Bcl-2, Bax and c caspase 3) were detected by Western blot. (f) The relative levels of proteins described in (e) were counted by GAPDH as normalization. ^aP < 0.05 vs. MC + NC, ^bP < 0.05 vs. MC + IGF1, ^cP < 0.05 vs. mimic+NC