Fig. 3

Impact of CPXM2 on the proliferation and metastasis of osteosarcoma cells in vitro. a Western blotting to examine the EMT process in the hFOB.1.19 cell line and the corresponding statistical analysis of EMT process components. b Growth curves of hFOB.1.19 cells as determined using the Cell Counting kit-8. c The abilities of hFOB.1.19 cells to form colonies under 2D culture condition were determined through colony formation assay. d Matrigel invasion assay to examine the invasive ability of hFOB.1.19 cells in vitro. Scale bars, 20 μm. e Wound-healing assay to detect the migration ability of hFOB.1.19 cells in vitro. ^**P < 0.01 vs. the empty vector groups. CPXM2, Carboxypeptidase X, M14 family member 2; EMT, epithelial mesenchymal transition; OD, optical density