Fig. 1

Interaction of miR-32-5p with KLF2 and its effect on HUVECs proliferation. a. The binding sequence of miR-32-5p in the 3′-UTR of KLF2. b. Expression of miR-32-5p was successfully upregulated by the miR-32-5p mimic, and was downregulated by the miR-32-5p inhibitor. c. The relative luciferase activity in HUVECs with overexpression or knockdown of miR-32-5p. d. HUVECs proliferation was promoted by the downregulation of miR-32-5p, but was inhibited by the overexpression of miR-32-5p. No significant difference was found in miR-32-5p expression, relative luciferase activity and cell proliferation between mock group and mimic NC or inhibitor NC group, which excluded the effect of transfection operations on the detected results. KLF2, Kruppel-like factor 2; 3′-UTR, 3′-untranslated region; NC, negative control; WT, wild type; MT, mutant type; *P < 0.05, **P < 0.01, ***P < 0.001