Potential utility of targeted Nanopore sequencing for improving etiologic diagnosis of bacterial and fungal respiratory infection

Table 2 PCR primers for bacterial 16S, fungal ITS and human beta-globin PCR

Primers	Sequences (5′ to 3′)	References
16S (27F)	AGAGTTTGATCMTGGCTCAG	[12]
16S (533F)	GTGCCAGCMGCCGCGGTAA	[13]
16S (1100R)	AGGGTTGCGCTCGTTG	[14]
16S (1492R)	TACGGYTACCTTGTTACGACTT	[12]
ITS5	GGAAGTAAAAGTCGTAACAAGG	[15]
ITS4	TCCTCCGCTTATTGATATGC	[15]
Human beta-globin forward (BG-F)	GAAGAGCCAAGGACAGGTAC	[16]
Human beta-globin reverse (BG-R)	GGAAAATAGACCAATAGGCAG	[16]

ISSN: 1746-1596