Table 5 Estimated reagent cost of Nanopore workflow (24-plex for MinION; 1 specimen for Flongle)
Reagent cost/ service charge per specimen (USD) | ||||
|---|---|---|---|---|
Nanopore | Send-out for pneumonia testing | FDA-cleared pneumonia panel (33 targets) | ||
MinION (23 specimens + 1 NC) | Flongle (1 specimen + 1 NC) | |||
DNA extraction | 13.44 | 25.76 | / | / |
PCR | 2.04 | 3.91 | / | / |
Library preparation& sequencing | 53.39 | 188.56 | / | / |
Total | 68.87 | 218.23 | 514.84 | 321.77 |