Fig. 3

CircKIF4A interacted with miR-152 and negatively regulated its expression. (a) The putative binding sequences between circKIF4A and miR-152 were shown. (b and c) The association between circKIF4A and miR-152 was analyzed using dual-luciferase reporter assay. (d and e) RIP and qRT-PCR assays were performed to measure the binding efficiency of circKIF4A and miR-152 to Ago2 or IgG in MCF-7 and MDA-MB-231 cells. (f and g) The expression of miR-152 in BC tissues and cells was determined by qRT-PCR. (h and i) The level of miR-152 in BC cells transfected with pcDNA-circKIF4A or si-circKIF4A or their controls was detected by qRT-PCR. ***P < 0.001