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Table 9 Comparison of estimated reagent cost of Nanopore workflow (24-plex) and randomly-selected prices of HPV genotyping referral service in Hong Kong

From: An economical Nanopore sequencing assay for human papillomavirus (HPV) genotyping

This study

Procedure

Number of specimens

Cost

DNA extraction and PCRs

201 patients +20 controls = 221

USD 20.02 × 221 reactions = USD 4424.42

Nanopore sequencing

115 patients / 24 = at least 5 runs

N = 120 for 1 positive control per run

USD 1155.94 × 5 runs = USD 5779.70

Cost per patient specimen

(4424.42 + 5779.70) / 201 = USD 50.77

Referral service (transportation cost not included)

Lab A

USD 77.19

Lab B

USD 124.79

Lab C

USD 101.63

Lab D

USD 120.93

Average

USD 106.14