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Table 1 RNA concentration and quality scores in the collected samples

From: Comparison of skin biopsy sample processing and storage methods on high dimensional immune gene expression using the Nanostring nCounter system

Method

Concentration

A260 / A280

Concentration

RQS

nCounter

NanoDrop (ng/μL)

Quantifluor (ng/μL)

counts

LN1

315.48

1.9

67.2

6.5

ok

LN2

106.69

1.83

86.4

4.8

ok

LN3

19.44

2.47

0.2

NA

NA

LN4

90.22

1.52

1.7

NA

ND

RL1

88.83

2.11

69.5

9.4

ok

RL2

68.38

2.06

40.2

8.1

ok

RL3

15.6

2.44

1.6

7.1

ND

RL4

9.01

3.3

ND

ND

NA

Q_FFPE1

68.02

1.63

13

5.3

ok

Q_FFPE2

63.62

1.74

25.6

5.4

ok

Q_FFPE3

30.13

1.94

16

5.6

ok

Q_FFPE4

36.21

1.82

13.2

5.9

ok

P-FFPE1

24.44

1.89

11.0

4.5

ok

P-FFPE2

49.53

1.79

19.0

4.4

ok

P-FFPE3

48.61

1.98

35.9

4

ok

P-FFPE4

45.24

1.87

20.8

3.2

NA

TR1

0.79

1.5

49.4

4.6

ok

TR2

1.91

2.55

37.9

4.6

ok

TR3

0.1

0.21

12

NA

ok

TR4

0

0.68

16.7

4.9

ok

RS1

20.84

2.88

34.6

4.2

ok

RS2

9.12

3.32

11

5.5

ok

RS3

3.65

−3.43

4.5

6.2

ok

RS4

19.34

2.49

4.8

5.3

NA

S1

127.41

1.59

1.8

ND

ND

S2

21.12

2.23

3.5

2.1

ND

S3

11.87

1.76

ND

ND

ND

S4

93.72

1.53

ND

ND

NA

  1. Processing and storage methods: LN- liquid nitrogen; RL – RNAlater; FFPE – formalin fixed paraffin embedded; TR – trizol; RS – RNA/DNA shield; S – saline; ng – nanograms; μL – microlitres; RQS – RNA integrity number. ND - under limit of detection; NA – sample was not run on nCounter. Quantifluor and RQS data was acquired after RNA concentration with.
  2. Zymo RNA Concentrator kit (Zymo).
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Diagnostic Pathology

ISSN: 1746-1596

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