| Method | Concentration | A260 / A280 | Concentration | RQS | nCounter |
|---|
| NanoDrop (ng/μL) | Quantifluor (ng/μL) | counts |
|---|
| LN1 | 315.48 | 1.9 | 67.2 | 6.5 | ok |
| LN2 | 106.69 | 1.83 | 86.4 | 4.8 | ok |
| LN3 | 19.44 | 2.47 | 0.2 | NA | NA |
| LN4 | 90.22 | 1.52 | 1.7 | NA | ND |
| RL1 | 88.83 | 2.11 | 69.5 | 9.4 | ok |
| RL2 | 68.38 | 2.06 | 40.2 | 8.1 | ok |
| RL3 | 15.6 | 2.44 | 1.6 | 7.1 | ND |
| RL4 | 9.01 | 3.3 | ND | ND | NA |
| Q_FFPE1 | 68.02 | 1.63 | 13 | 5.3 | ok |
| Q_FFPE2 | 63.62 | 1.74 | 25.6 | 5.4 | ok |
| Q_FFPE3 | 30.13 | 1.94 | 16 | 5.6 | ok |
| Q_FFPE4 | 36.21 | 1.82 | 13.2 | 5.9 | ok |
| P-FFPE1 | 24.44 | 1.89 | 11.0 | 4.5 | ok |
| P-FFPE2 | 49.53 | 1.79 | 19.0 | 4.4 | ok |
| P-FFPE3 | 48.61 | 1.98 | 35.9 | 4 | ok |
| P-FFPE4 | 45.24 | 1.87 | 20.8 | 3.2 | NA |
| TR1 | 0.79 | 1.5 | 49.4 | 4.6 | ok |
| TR2 | 1.91 | 2.55 | 37.9 | 4.6 | ok |
| TR3 | 0.1 | 0.21 | 12 | NA | ok |
| TR4 | 0 | 0.68 | 16.7 | 4.9 | ok |
| RS1 | 20.84 | 2.88 | 34.6 | 4.2 | ok |
| RS2 | 9.12 | 3.32 | 11 | 5.5 | ok |
| RS3 | 3.65 | −3.43 | 4.5 | 6.2 | ok |
| RS4 | 19.34 | 2.49 | 4.8 | 5.3 | NA |
| S1 | 127.41 | 1.59 | 1.8 | ND | ND |
| S2 | 21.12 | 2.23 | 3.5 | 2.1 | ND |
| S3 | 11.87 | 1.76 | ND | ND | ND |
| S4 | 93.72 | 1.53 | ND | ND | NA |
- Processing and storage methods: LN- liquid nitrogen; RL – RNAlater; FFPE – formalin fixed paraffin embedded; TR – trizol; RS – RNA/DNA shield; S – saline; ng – nanograms; μL – microlitres; RQS – RNA integrity number. ND - under limit of detection; NA – sample was not run on nCounter. Quantifluor and RQS data was acquired after RNA concentration with.
- Zymo RNA Concentrator kit (Zymo).
