Skip to main content

Table 2 Immune gene expression counts above background, below 100, between 100 and 1000 and above 1000 by mRNA extraction method. Values presented are mean counts ± standard deviation of the four processing storage methods. No counts could be determined from the saline samples

From: Comparison of skin biopsy sample processing and storage methods on high dimensional immune gene expression using the Nanostring nCounter system

 

FFPE

LN

TR

RS

RLT

Above background

730

663.5 ± 53.03

719.67 ± 8.96

730

579.50 ± 65.76

Below 100

494 ± 8.28

523 ± 25.45

504 ± 13.45

514.75 ± 4.5

547.5 ± 9.19

between 100 and 1000

199.25 ± 9.46

174.5 ± 17.67

193.33 ± 14.01

180.5 ± 16.7

155 ± 9.89

above 1000

36.75 ± 1.5

32.5 ± 7.77

32.66 ± 0.57

34.75 ± 4.34

27.5 ± 0.7

  1. Processing and storage methods: LN- liquid nitrogen; RL – RNAlater; FFPE – formalin fixed paraffin embedded; TR – trizol; RS – RNA / DNA shield;