Fig. 4

The effect of miR-142-5p on hippocampal neuron cell viability and apoptosis. a MiR-142-5p inhibitor transfection significantly reduced miR-142-5p level. b Isoflurane treatment significantly increased the expression level of miR-142-5p in hippocampal neurons, but miR-142-5p inhibitor transfection significantly reversed the effect. c MTT assay results suggested that isoflurane treatment inhibited the cell viability significantly, which was alleviated by miR-142-5p downregulation. d Flow cytometry assay results demonstrated that isoflurane treatment promoted cell apoptosis, while miR-142-5p downregulation relieved isoflurane-induced cell apoptosis. *** P < 0.001, compared with control group; ^#P < 0.05, ^###P < 0.001, compared with isoflurane group