Fig. 1

Western blot and immunohistochemical expression of FAM83H and ZNF16 and statistical analysis in gallbladder carcinomas. a SNU-308 gallbladder carcinoma cells were transfected with control shRNA, shRNA for FAM83H, empty vector, or a vector overexpressing FAM83H (FAM83H-OE) and performed western blot for FAM83H, ZNF16, and actin. b FAM83H and ZNF16 are expressed in both the cytoplasm and nuclei of cancer cells. Original magnification: × 400. c Receiver operating characteristic curve analysis to determine cut-off points for the expression of nuclear FAM83H (blue arrowhead), cytoplasmic FAM83H (empty blue arrowhead), nuclear ZNF16 (red arrow), and cytoplasmic ZNF16 (empty red arrow). The cut-off points indicate the point of the highest area under the curve (AUC) to predict the death of gallbladder carcinoma patients